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KMID : 0191120000150050521
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Journal of Korean Medical Science
2000 Volume.15 No. 5 p.521 ~ p.528
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Effects of prednisolone on eosinophils, IL-5, eosinophil cationic protein, EG2+ eosinophils, and nitric oxide metabolites in the sputum of patients with exacerbated asthma
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Jang AS
Choi IS/Koh YI/Jeong TK/Lee KY/Kim YS/Lee JU/Park CS
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Abstract
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Corticosteroids are considered to be one of the most effective medicine for asthma by suppressing
airway inflammation. This study was carried out to investigate the effects of prednisolone in the
sputum of exacerbated asthmatics. Clinical severity, cell differentials, levels of interleukin
(IL)-5, eosinophil cationic protein (ECP), EG2+ eosinophils, and nitric oxide (NO) metabolites
were measured. Sputum was examined 2 weeks apart in 13 exacerbated asthmatics before and after
prednisolone treatment, and once in 12 stable asthmatics. We used a sandwich ELISA for IL-5,
fluoroimmunoassay for ECP, immunohistochemical staining for EG2+ eosinophils, a NO metabolites
assay using modified Griess reaction. Exacerbated asthmatics, in comparison with stable asthmatics,
had significantly higher proportion of eosinophils, higher level of ECP, higher percentage of EG2+
eosinophils, and NO metabolites. Exacerbated asthmatics after treatment with prednisolone had
reduced the proportions of eosinophils, reduced level of IL-5, ECP and percentage of EG2+
eosinophils. FEV1 was correlated with the proportion of eosinophils, ECP, and IL-5 respectively.
These findings suggest that prednisolone is considered to be effective medicine for asthma by
suppressing eosinophil activation through IL-5.
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